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Position Summary

Our client is seeking an experienced, skilled, and creative contract biologist, virologist, or bioengineer to lead discovery and engineering campaigns focused on next-generation gene therapies for rare diseases. The successful candidate will operate within a cross-disciplinary team of experimental and computational scientists, conducting wet lab experiments in support of programs.

Responsibilities

• Lead biological engineering projects focused on development of next-generation viral vectors
• Independently design, optimize, and execute high-throughput molecular and cellular screening systems to identify and characterize novel viral capsids.
• Support viral vector projects, including transfection, transduction, viral titering, and sequencing-based characterization of viral genomes.
• Implement advanced molecular biology methods to design and build high diversity viral vector libraries
• Design, optimize, and execute molecular biology workflows, including DNA/RNA extraction, cloning and PCR/qPCR/ddPCR
• Prepare Next-Generation Sequencing (NGS) libraries (Illumina, Nanopore, PacBio) and perform quality measurements (qubit, tape station).
• Perform cell culture with mammalian cell lines and primary cells
• Design, conduct, and support in vivo studies
• Perform data analysis and review, ensuring accuracy and completeness
• Document protocols, write reports, and present findings to cross-disciplinary teams.
• Contribute to laboratory operations, including training of junior staff and maintenance of high-quality research standards.

Requirements

• PhD in cell biology, virology, immunology, molecular biology, bioengineering, or related field with 2-6+ years of relevant industry experience or MS with 8+ years of relevant hands-on experience.
• Direct report or small team management experience strongly preferred. Position commensurate with experience.
• Proven track record in method development, including experimental design, optimization, execution, and evaluation.
• Experience with viruses or viral vectors required; experience with AAV vectors strongly preferred.
• Experience with viral transfection, transduction, ultracentrifugation purification (CsCl, iodixanol), genome titer (by qPCR, ddPCR, RT-qPCR, RT-ddPCR) and characterization required.
• Experience with viral vector engineering (large library preparation and screening) required.
• Experience working with mammalian cell lines and primary cells required.
• Hands-on in vivo model or study management experience preferred.
• Strong background in advanced molecular biology methods including cloning and recombinant DNA technologies (DNA/RNA extraction, primer/probe design, PCR/qPCR/ddPCR, Gibson Assembly, Golden Gate ect.) preferred.
• Proficiency in NGS technologies (Illumina, Nanopore, PacBio), library preparation, and quality assessment (qubit, tape station) preferred.
• Experience with cell-based functional assays including but not limited to ELISpot, ICS, flow cytometry, antibody-neutralization assays, and potency assays preferred.
• Experience with protein characterization methods, including but not limited to ELISA, SDS-PAGE, western blot, TFF, and affinity chromatography preferred.
• Strong written and oral communication skills are required.
• Applicants should be detail-oriented, highly organized, and able to multi-task with multiple projects.
• Strong analytical and problem-solving skills a must

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